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anti jag1 apc  (R&D Systems)


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    R&D Systems anti jag1 apc
    Anti Jag1 Apc, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti jag1 apc/product/R&D Systems
    Average 90 stars, based on 2 article reviews
    anti jag1 apc - by Bioz Stars, 2026-03
    90/100 stars

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    anti jag1 apc  (R&D Systems)
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    R&D Systems anti jag1 apc
    Anti Jag1 Apc, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti jag1 apc/product/R&D Systems
    Average 90 stars, based on 1 article reviews
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    jag1 apc  (R&D Systems)
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    Cellular composition switch of freshly isolated human dental pulp stem cells (hDPSCs) upon in vitro culture. a UMAP visualization of monolayer cultured cells (red dots) compared to the freshly isolated cells (blue dots). The purple dash circles highlighted the most overlapped zone in both conditions. b UMAP visualization of 14 color-coded clusters of integrated freshly isolated and 10-day in vitro cultured hDPSCs. Four major cell types are identified, including dental pulp cells, endothelia cells, immune cells, and glial cells. Cluster 4 cells present in both datasets. c Differentially expressed genes in cluster 4 from fresh and cultured datasets were listed in the blue and pink circles, respectively. The overlapped gray region highlights the genes with similar expression levels in cluster 4 from both datasets. Expression dynamics along the pseudotime of selected genes from left panel was plotted by Monocle3. d The distribution (left) and quantification (right) of selected stem cell surface makers genes (MCAM, <t>JAG1,</t> and PDGFRA) expression in both cultured and fresh hDPSCs. Cells with low and high expression were marked with gray and purple color, respectively
    Jag1 Apc, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/jag1 apc/product/R&D Systems
    Average 90 stars, based on 1 article reviews
    jag1 apc - by Bioz Stars, 2026-03
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    Cellular composition switch of freshly isolated human dental pulp stem cells (hDPSCs) upon in vitro culture. a UMAP visualization of monolayer cultured cells (red dots) compared to the freshly isolated cells (blue dots). The purple dash circles highlighted the most overlapped zone in both conditions. b UMAP visualization of 14 color-coded clusters of integrated freshly isolated and 10-day in vitro cultured hDPSCs. Four major cell types are identified, including dental pulp cells, endothelia cells, immune cells, and glial cells. Cluster 4 cells present in both datasets. c Differentially expressed genes in cluster 4 from fresh and cultured datasets were listed in the blue and pink circles, respectively. The overlapped gray region highlights the genes with similar expression levels in cluster 4 from both datasets. Expression dynamics along the pseudotime of selected genes from left panel was plotted by Monocle3. d The distribution (left) and quantification (right) of selected stem cell surface makers genes (MCAM, JAG1, and PDGFRA) expression in both cultured and fresh hDPSCs. Cells with low and high expression were marked with gray and purple color, respectively

    Journal: International Journal of Oral Science

    Article Title: Single-cell characterization of monolayer cultured human dental pulp stem cells with enhanced differentiation capacity

    doi: 10.1038/s41368-021-00140-6

    Figure Lengend Snippet: Cellular composition switch of freshly isolated human dental pulp stem cells (hDPSCs) upon in vitro culture. a UMAP visualization of monolayer cultured cells (red dots) compared to the freshly isolated cells (blue dots). The purple dash circles highlighted the most overlapped zone in both conditions. b UMAP visualization of 14 color-coded clusters of integrated freshly isolated and 10-day in vitro cultured hDPSCs. Four major cell types are identified, including dental pulp cells, endothelia cells, immune cells, and glial cells. Cluster 4 cells present in both datasets. c Differentially expressed genes in cluster 4 from fresh and cultured datasets were listed in the blue and pink circles, respectively. The overlapped gray region highlights the genes with similar expression levels in cluster 4 from both datasets. Expression dynamics along the pseudotime of selected genes from left panel was plotted by Monocle3. d The distribution (left) and quantification (right) of selected stem cell surface makers genes (MCAM, JAG1, and PDGFRA) expression in both cultured and fresh hDPSCs. Cells with low and high expression were marked with gray and purple color, respectively

    Article Snippet: Each of the following conjugated anti-human monoclonal antibodies were incubated with the cell suspensions at 37 °C for 30 min in the dark: MCAM/PE (BD 550315), JAG1/APC (R&D FAB1277A) and PDGFRa/BB700 (BD 746023).

    Techniques: Isolation, In Vitro, Cell Culture, Expressing

    Distribution of MCAM(+)JAG1(+)PDGFRA(−) cells in the extracted human third molar. a Immunofluorescence double staining of endothelial cells markers CD31 (red) with MCAM (green, top) and JAG1 (green, bottom), respectively. b Immunofluorescence double staining of MCAM (red) and JAG1 (green) c Immunofluorescence double staining of PDGFRA (red) with MCAM (green) and JAG1 (green), respectively. The odontoblast layer was indicated by the dash lines, and the magnified area (dotted boxes) was shown in yellow dotted boxes (the middle panels). Nucleus were labeled with DAPI (blue). White arrowheads indicated characteristic blood vessel structures. Scale bars = 50 μm

    Journal: International Journal of Oral Science

    Article Title: Single-cell characterization of monolayer cultured human dental pulp stem cells with enhanced differentiation capacity

    doi: 10.1038/s41368-021-00140-6

    Figure Lengend Snippet: Distribution of MCAM(+)JAG1(+)PDGFRA(−) cells in the extracted human third molar. a Immunofluorescence double staining of endothelial cells markers CD31 (red) with MCAM (green, top) and JAG1 (green, bottom), respectively. b Immunofluorescence double staining of MCAM (red) and JAG1 (green) c Immunofluorescence double staining of PDGFRA (red) with MCAM (green) and JAG1 (green), respectively. The odontoblast layer was indicated by the dash lines, and the magnified area (dotted boxes) was shown in yellow dotted boxes (the middle panels). Nucleus were labeled with DAPI (blue). White arrowheads indicated characteristic blood vessel structures. Scale bars = 50 μm

    Article Snippet: Each of the following conjugated anti-human monoclonal antibodies were incubated with the cell suspensions at 37 °C for 30 min in the dark: MCAM/PE (BD 550315), JAG1/APC (R&D FAB1277A) and PDGFRa/BB700 (BD 746023).

    Techniques: Immunofluorescence, Double Staining, Labeling

    Flow-cytometry analysis of MCAM( + )JAG1( + ) PDGFRA(−) cells in human dental pulps from young, aged, healthy, and dental caries samples. a Representative graphs of dental pulp cell suspensions gained from young, following exclusion of PDGFRA(+) cells (purple boxes) and selection of double positive of JAG1 and MCAM cells (blue boxes). b Representative graphs of dental pulp cell suspensions gained from aged samples. c The percentage of MCAM(+)JAG1(+) PDGFRA(−) cells (blue numbers) in young/aged groups showed no significant differences by two-tailed t test. d Representative graphs of dental pulp cell suspensions gained from healthy samples. e Representative graphs of dental pulp cell suspensions gained from dental caries samples. f The percentage of MCAM(+)JAG1(+)PDGFRA(−) cells (blue numbers) in healthy and dental caries groups shows no significant differences by two-tailed t test

    Journal: International Journal of Oral Science

    Article Title: Single-cell characterization of monolayer cultured human dental pulp stem cells with enhanced differentiation capacity

    doi: 10.1038/s41368-021-00140-6

    Figure Lengend Snippet: Flow-cytometry analysis of MCAM( + )JAG1( + ) PDGFRA(−) cells in human dental pulps from young, aged, healthy, and dental caries samples. a Representative graphs of dental pulp cell suspensions gained from young, following exclusion of PDGFRA(+) cells (purple boxes) and selection of double positive of JAG1 and MCAM cells (blue boxes). b Representative graphs of dental pulp cell suspensions gained from aged samples. c The percentage of MCAM(+)JAG1(+) PDGFRA(−) cells (blue numbers) in young/aged groups showed no significant differences by two-tailed t test. d Representative graphs of dental pulp cell suspensions gained from healthy samples. e Representative graphs of dental pulp cell suspensions gained from dental caries samples. f The percentage of MCAM(+)JAG1(+)PDGFRA(−) cells (blue numbers) in healthy and dental caries groups shows no significant differences by two-tailed t test

    Article Snippet: Each of the following conjugated anti-human monoclonal antibodies were incubated with the cell suspensions at 37 °C for 30 min in the dark: MCAM/PE (BD 550315), JAG1/APC (R&D FAB1277A) and PDGFRa/BB700 (BD 746023).

    Techniques: Flow Cytometry, Selection, Two Tailed Test

    In vitro cellular performance of MCAM( + )JAG1( + ) PDGFRA(−) hDPSCs. a MCAM(+)JAG1(+) PDGFRA(−) and PDGFRA(+) hDPSCs were sorted by FACS. b Contrast microscopic images of cellular spheres (diameter exceeds 25 μm) formed by MCAM(+)JAG1(+) PDGFRA(−) and PDGFRA(+) hDPSCs, Scale bar = 100 μm; and the quantification of the spheres from each group formed after 7 days. * P < 0.05. c In vitro proliferation of MCAM(+)JAG1(+)PDGFRA(−) and PDGFRA(+) hDPSCs during 6-day culture. * P < 0.05. d Osteogenic marker genes expressions of RUNX2, COL1A1 , and BSP of MCAM(+)JAG1(+)PDGFRA(−) and PDGFRA(+) hDPSCs after 14-day culture. e Western blot and quantitative analysis of RUNX2 expression in protein level after 7 days induction. f Alizarin Red S staining and quantification of the mineralized matrix obtained from MCAM(+)JAG1(+)PDGFRA(−) and PDGFRA(+) hDPSCs after 21 day culture in osteogenic medium

    Journal: International Journal of Oral Science

    Article Title: Single-cell characterization of monolayer cultured human dental pulp stem cells with enhanced differentiation capacity

    doi: 10.1038/s41368-021-00140-6

    Figure Lengend Snippet: In vitro cellular performance of MCAM( + )JAG1( + ) PDGFRA(−) hDPSCs. a MCAM(+)JAG1(+) PDGFRA(−) and PDGFRA(+) hDPSCs were sorted by FACS. b Contrast microscopic images of cellular spheres (diameter exceeds 25 μm) formed by MCAM(+)JAG1(+) PDGFRA(−) and PDGFRA(+) hDPSCs, Scale bar = 100 μm; and the quantification of the spheres from each group formed after 7 days. * P < 0.05. c In vitro proliferation of MCAM(+)JAG1(+)PDGFRA(−) and PDGFRA(+) hDPSCs during 6-day culture. * P < 0.05. d Osteogenic marker genes expressions of RUNX2, COL1A1 , and BSP of MCAM(+)JAG1(+)PDGFRA(−) and PDGFRA(+) hDPSCs after 14-day culture. e Western blot and quantitative analysis of RUNX2 expression in protein level after 7 days induction. f Alizarin Red S staining and quantification of the mineralized matrix obtained from MCAM(+)JAG1(+)PDGFRA(−) and PDGFRA(+) hDPSCs after 21 day culture in osteogenic medium

    Article Snippet: Each of the following conjugated anti-human monoclonal antibodies were incubated with the cell suspensions at 37 °C for 30 min in the dark: MCAM/PE (BD 550315), JAG1/APC (R&D FAB1277A) and PDGFRa/BB700 (BD 746023).

    Techniques: In Vitro, Marker, Western Blot, Expressing, Staining

    In vitro cellular performance of MCAM(+)JAG1(+) PDGFRA(−) hDPSCs. a Chondrogenic marker gene expression of SOX9, ACAN, and COL2A1 of MCAM(+)JAG1(+)PDGFRA(−) and PDGFRA(+) hDPSCs after 14-day culture. b Western blot and quantitative analysis of SOX9 expression in protein level after 7 days induction. c Alcian blue staining and quantification of the cultured pellets obtained from MCAM(+)JAG1(+)PDGFRA(−) and PDGFRA(+) hDPSCs after 28 days induction. d Adipogenic marker gene expression of PPARG, FABP4 , and CEBPA of MCAM(+)JAG1(+)PDGFRA(−) and PDGFRA(+) hDPSCs after 14-day culture. e Western blot and quantitative analysis of PPARG expression in protein level after 14 days induction. f Oil red O staining and quantification of the adipocytes obtained from MCAM(+)JAG1(+)PDGFRA(−) and PDGFRA(+) hDPSCs after 28 days induction. GAPDH was used as the normalization control in qPCR. The levels of ACTIN are used as loading control in western blot. Bars represent mean ± SD values. * P < 0.05; ** P < 0.01; *** P < 0.001

    Journal: International Journal of Oral Science

    Article Title: Single-cell characterization of monolayer cultured human dental pulp stem cells with enhanced differentiation capacity

    doi: 10.1038/s41368-021-00140-6

    Figure Lengend Snippet: In vitro cellular performance of MCAM(+)JAG1(+) PDGFRA(−) hDPSCs. a Chondrogenic marker gene expression of SOX9, ACAN, and COL2A1 of MCAM(+)JAG1(+)PDGFRA(−) and PDGFRA(+) hDPSCs after 14-day culture. b Western blot and quantitative analysis of SOX9 expression in protein level after 7 days induction. c Alcian blue staining and quantification of the cultured pellets obtained from MCAM(+)JAG1(+)PDGFRA(−) and PDGFRA(+) hDPSCs after 28 days induction. d Adipogenic marker gene expression of PPARG, FABP4 , and CEBPA of MCAM(+)JAG1(+)PDGFRA(−) and PDGFRA(+) hDPSCs after 14-day culture. e Western blot and quantitative analysis of PPARG expression in protein level after 14 days induction. f Oil red O staining and quantification of the adipocytes obtained from MCAM(+)JAG1(+)PDGFRA(−) and PDGFRA(+) hDPSCs after 28 days induction. GAPDH was used as the normalization control in qPCR. The levels of ACTIN are used as loading control in western blot. Bars represent mean ± SD values. * P < 0.05; ** P < 0.01; *** P < 0.001

    Article Snippet: Each of the following conjugated anti-human monoclonal antibodies were incubated with the cell suspensions at 37 °C for 30 min in the dark: MCAM/PE (BD 550315), JAG1/APC (R&D FAB1277A) and PDGFRa/BB700 (BD 746023).

    Techniques: In Vitro, Marker, Expressing, Western Blot, Staining, Cell Culture

    Osteogenic differentiation of MCAM( + )JAG1( + )PDGFRA(−) and PDGFRA( + ) hDPSCs in vivo. a Representative images of HE staining from cell-laden constructs after 4 weeks subcutaneous implantation. S scaffolds, B newly-formed bone tissues. Scale bars = 50 μm. b Representative images of immunohistochemical staining of human mitochondria. Scale bars = 100 μm. c Representative images of immunohistochemical staining of human specific human specific osteocalcin (OCN). Scale bars = 100 μm

    Journal: International Journal of Oral Science

    Article Title: Single-cell characterization of monolayer cultured human dental pulp stem cells with enhanced differentiation capacity

    doi: 10.1038/s41368-021-00140-6

    Figure Lengend Snippet: Osteogenic differentiation of MCAM( + )JAG1( + )PDGFRA(−) and PDGFRA( + ) hDPSCs in vivo. a Representative images of HE staining from cell-laden constructs after 4 weeks subcutaneous implantation. S scaffolds, B newly-formed bone tissues. Scale bars = 50 μm. b Representative images of immunohistochemical staining of human mitochondria. Scale bars = 100 μm. c Representative images of immunohistochemical staining of human specific human specific osteocalcin (OCN). Scale bars = 100 μm

    Article Snippet: Each of the following conjugated anti-human monoclonal antibodies were incubated with the cell suspensions at 37 °C for 30 min in the dark: MCAM/PE (BD 550315), JAG1/APC (R&D FAB1277A) and PDGFRa/BB700 (BD 746023).

    Techniques: In Vivo, Staining, Construct, Immunohistochemical staining

    Adipogenic differentiation of MCAM( + )JAG1( + )PDGFRA(−) and PDGFRA( + ) hDPSCs in vivo. a Representative images of HE staining from cell-laden constructs when transplanted into immunocompromised mice for 4 weeks. A higher magnification emphasizing the yellow dotted portions. Scale bars = 200 μm. b Representative images of immunohistochemical staining of human specific mitochondria. Scale bars = 100 μm

    Journal: International Journal of Oral Science

    Article Title: Single-cell characterization of monolayer cultured human dental pulp stem cells with enhanced differentiation capacity

    doi: 10.1038/s41368-021-00140-6

    Figure Lengend Snippet: Adipogenic differentiation of MCAM( + )JAG1( + )PDGFRA(−) and PDGFRA( + ) hDPSCs in vivo. a Representative images of HE staining from cell-laden constructs when transplanted into immunocompromised mice for 4 weeks. A higher magnification emphasizing the yellow dotted portions. Scale bars = 200 μm. b Representative images of immunohistochemical staining of human specific mitochondria. Scale bars = 100 μm

    Article Snippet: Each of the following conjugated anti-human monoclonal antibodies were incubated with the cell suspensions at 37 °C for 30 min in the dark: MCAM/PE (BD 550315), JAG1/APC (R&D FAB1277A) and PDGFRa/BB700 (BD 746023).

    Techniques: In Vivo, Staining, Construct, Immunohistochemical staining